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INTENDED USE AND TEST PRINCIPLE
This PIGR Ab ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of PIGR Ab in the sample, this PIGR Ab ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus PIGR Ab concentration. The concentration of PIGR Ab in the samples is then determined by comparing the O.D. of the samples to the standard curve.
This PIGR Ab ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of PIGR Ab in the sample, this PIGR Ab ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus PIGR Ab concentration. The concentration of PIGR Ab in the samples is then determined by comparing the O.D. of the samples to the standard curve.
SAMPLE COLLECTION AND STORAGES
Serum - Use a serum separator tube and allow samples to clot for two hours at room temperature or
overnight at 4℃ before centrifugation for 20 minutes at approximately 1000×g. Assay freshly
prepared serum immediately or store samples in aliquot at -20℃ or -80℃ for later use. Avoid
repeated freeze/thaw cycles.
Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15
minutes at 1000×g at 2-8 ℃ within 30 minutes of collection. Remove plasma and assay
immediately or store samples in aliquot at -20 ℃ or -80 ℃ for later use. Avoid repeated
freeze/thaw cycles.
Tissue homogenates - For general information, hemolysis blood may affect the result, so you
should rinse the tissues with ice-cold PBS (0.01M, pH=7.4) to remove excess blood thoroughly. Tissue pieces should be weighed and then minced to small pieces which will be homogenized
in PBS (the volume depends on the weight of the tissue. 9mL PBS would be appropriate to 1
gram tissue pieces. Some protease inhibitor is recommended to add into the PBS.) with a glass
homogenizer on ice. To further break the cells, you can sonicate the suspension with an
ultrasonic cell disrupter or subject it to freeze-thaw cycles. The homogenates are then
centrifugated for 5minutes at 5000×g to get the supernate.
Cell culture supernates and other biological fluids - Centrifuge samples for 20 minutes at