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PRODUCT INFORMATION
Contents
Plasmocin® Prophylactic is supplied as a cell culture tested, sterile
filtered yellow solution at 2.5 mg/ml.
• ant-mpp:
10 x 1 ml (25 mg)
One 1 ml vial is sufficient for 500 ml of culture.
Storage and stability
- Plasmocin® Prophylactic is shipped at room temperature. Upon
receipt it can be stored at 4°C for 1 month or at -20°C for long-term
storage. Avoid repeated freeze-thaw cycles.
- The expiry date is specified on the product label.
Note: Product is stable for 2 weeks at room temperature.
QUALITY CONTROL
Each lot is thoroughly tested to ensure the absence of lot-to-lot
variation:
- Endotoxin level: < 10 EU/mg
- Physicochemical characterization (pH, appearance)
- Cell culture tested: potency validated on bacterial reference strains.
BACKGROUND
Mycoplasma contamination is a significant problem for mammalian
cell culture. Reports estimate mycoplasma contamination in up
to 35% of all cell cultures. Unlike bacterial or fungal contaminations,
mycoplasma cannot be detected by visual inspection and may not
noticeably affect cell culture growth rates. However, mycoplasma
infection has been shown to alter DNA, RNA and protein synthesis,
introduce chromosomal aberrations and cause alterations or
modifications of host cell plasma membrane antigens.
DESCRIPTION
Plasmocin® Prophylactic is a highly cited broad-spectrum
anti-mycoplasma reagent. It can be used as a “routine addition”
in liquid media to prevent mycoplasma and related cell wall-less
bacteria contamination in mammalian cell cultures. It is active
against numerous mycoplasmas, such as M. arginini, M. fermentans,
M. laidlawii, and M. hyorhinis. In contrast to other anti-mycoplasma
compounds, Plasmocin® is active on both extracellular mycoplasmas
and intracellular forms. This advantage is conferred by one component
of Plasmocin® that is actively transported into mammalian cells.
Plasmocin® is active at low concentrations on a broad range of
Gram-positive bacteria, such as Staphylococcus species, and Gram
negative bacteria, such as E. coli, Enterobacter, Pseudomonas, and
Alcaligenes. Plasmocin® Prophylactic (5 µg/ml) exhibits no toxicity
in eukaryotic cells. Its activity is unaffected by the presence of up
to 20% serum in cell culture medium.
