Introduction

1. This RIPA Lysis Buffer is a kind of traditional cell and tissue lysis buffer. The end protein / sample lysed by this buffer can be used for Western Blot and Immunoprecipitation.

2. RIPA (Radio Immunoprecipitation Assay) Lysis Buffer has different formulas, and according to its lysis strength, it can be roughly classified into three categories: strong, medium and weak.

3. RIPA Lysis Buffer (Medium) contains: 50mM Tris (pH 7.4), 150mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, and different kinds of inhibitors (sodium orthovanadate, sodium fluoride, EDTA and leupeptin), which can effectively inhibit the protein degradation.

4. The concentration of the protein which lysed by this buffer can be determined by our BCA Protein Assay Kits (Catalog # BWR1023, BWR1024, BWR1025, BWR1026). For the high concentration of detergent, it is not recommended to use Bradford Protein Assay Kit.