Size: 96T
Range: 15.6 pg/ml-1000 pg/ml
Sensitivity < 2 pg/ml
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Application: For quantitative detection of VEGF in mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Kit components
1. One 96-well plate pre-coated with anti-Mouse VEGF antibody
2. Lyophilized Mouse VEGF standards: 2 tubes (10 ng / tube)
3. Sample / Standard diluent buffer: 30ml
4. Biotin conjugated anti-Mouse VEGF antibody (Concentrated): 130μl. Dilution: 1:100
5. Antibody diluent buffer: 12ml
6. Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130μl. Dilution: 1:100
7. ABC diluent buffer: 12ml
8. TMB substrate: 10ml
9. Stop solution: 10ml
10. Wash buffer (25X): 30ml
Note: Reconstitute standards and test samples with Kit Component 3.