会员登录.getTime()%>){kind=logo}
购物车()
首页>
生物试剂
成功收藏产品
商家描述
产品评价(0)
官网https://www.ptgcn.com/products/HA-Tag-Antibody-66006-2-Ig.htm
Host / Isotype
Mouse / IgG1
Reactivity
recombinant protein and More (1)
Applications
WB, IF/ICC, IP, ELISA and More (5)
Conjugate
Unconjugated
252
CloneNo.
1F5C6
经过测试的应用
| Positive WB detected in | Transfected HEK-293 cells, HEK-293 cells, Ag19172 |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) |
WB : 1:10000-1:100000 |
| Immunoprecipitation (IP) |
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC |
IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
产品信息
66006-2-Ig targets HA Tag in WB, IF/ICC, IP, CoIP, ChIP, RIP, ELISA, PLA applications and shows reactivity with recombinant protein samples.
| Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IF, IP, CoIP, ChIP, RIP, ELISA, PLA |
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | HA Tag |
| Synonyms | HA Tag, YPYDVPDYA |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2881490 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.