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官网https://www.ptgcn.com/products/Flag-tag-Antibody-66008-4-Ig.htm
产品信息
66008-4-Ig targets DYKDDDDK tag in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with recombinant protein samples.
Tested Applications | WB, IF/ICC, FC (Intra), IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP, ChIP, RIP |
Tested Reactivity | Recombinant Protein |
Cited Reactivity | Human, Chicken |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | DYKDDDDK tag |
Synonyms | Flag tag, Flag, DYKDDDDK, DDDDK tag, DDDDK |
Gene Symbol | |
Gene ID (NCBI) | |
RRID | AB_2918475 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.