Target Substance P Target background The discovery of substance P (SP) was reported in 1931. After more than 70 years of investigation, SP is perhaps the best understood neuropeptide transmitter. Substance P is an undecapeptide, which by the mid-1980s was recognized to belong to the tachykinin peptide family; it is also member of the neurokinins. It has been proposed that SP, released from primary afferent nerve endings, plays a role in chronic inflammation and pain. Neurotransmitters appear to play a key role in the regulation of emotions and antagonists of their receptors may be novel psychotropic drugs of the future. Target alias Substance P, tachykinin, neuropeptide, neurokinin, SP, anti-tachykinin, anti-neuropeptide, anti-neurokinin, anti-SP Immunogen Substance P Specificity This antibody recognizes the COOH-terminal end of substance P. It does not recognize Leu- or Met enkephalin, somatostatin or beta-endorphin; cross-reactivity with eledoisin: 5%. It recognizes Substance P in post-mortem tissue of the human brain. Clone ID NC1/34 Isotype IgG2a Preservative 0.05% thimerosal (Thimerosal-free version, MM-1021) Format Lyophilized tissue culture supernatant Recommend starting dilution If reconstituted with deionized water in 0.5 mL: IHC and ICC 1:200 - 1:500. Optimal dilution has to be determined by the user. Limitations Research Use Only Storage Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay. References