Target Nerve growth Factor (NGF) Target background Nerve growth factor (NGF) is a small secreted protein which induces the differentiation and survival of particular target neurons. It is perhaps the prototypical growth factor; in that it is one of the first to be described. NGF is critical for the survival and maintenance of sympathetic and sensory neurons. NGF is released from the target cells, binds to and activates its high affinity receptor (TrkA), and is internalized into the responsive neuron. There is some data that shows that NGF can be transported from the axon tip to soma. One of the most important molecules in the nervous system, nerve growth factor and its other neurotrophin family members control the development of the nervous system in the embryo and the maintenance of nervous tissue and neural transmission in the adult. Target alias Beta-nerve growth factor, Beta-NGF, NGF Immunogen polymerized NGF Specificity This antibody recognizes mouse NGF monomer protein (MW 13 kDa) by WB. It does not cross react with EGF, BSA, APO or insulin. Clone ID NGF30 Isotype IgG2a Preservative None Format Purified with protein G, stored in PBS pH 7.4 and lyophilized. Recommend starting dilution If reconstituted with deionized water in 100 µL: WB 1:200. Optimal dilution has to be determined by the user. Limitations Research Use Only Storage Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay. References