Target Amyloid Beta Target background Progressive deposition of insoluble aggregates of the 39 to 43 amino acid amyloid β (Aβ) peptide derived from the proteolytic cleavage of the amyloid β-protein precursor (AβPP), gives rise to one of the pathological hallmarks of Alzheimer’s disease (AD). The formation of Aβ fragments is a critical determinant in unleashing AD neuropathology. Target alias Beta-amyloid, ABP, amyloid peptide Immunogen Full length Amyloid beta Specificity A highly specific and sensitive antibody for amyloid beta protein. The antibody recognize the synthetic soluble peptide in ELISA. Both neuritic and diffuse plaques can be detected as well as cerebrovascular amyloid in affected cortex of Alzheimer’s disease (AD) brain by ICC Clone ID McSA1 Isotype IgG1 kappa Preservative None Format Purified with protein G, stored in PBS pH 7.4 and lyophilized. Recommend starting dilution If reconstituted with deionized water in 100 µL: IHC / ICC 1:500. Optimal dilution has to be determined by the user. Limitations Research Use Only Storage Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.