Target Vglut1 Target background Glutamate is the major excitatory neurotransmitter in the central nervous system of mammals. Vesicular glutamate transporters (VGluts) are responsible for glutamate transport from the brain into native synaptic vesicles. The level of expression of the three known transporters in the adult brain determines the amount of glutamate that is loaded into vesicles and released. Target alias Vesicular glutamate transporter 1, VGluT1, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 7 Immunogen Full Length VGlut1 Specificity A highly specific and sensitive antibody for VGlut1.The antibody reveals the presence of the 58 kDa VGlut 1 protein on western blot of homogenized cerebral cortex cells lysates. It shows very precise reaction with VGlut1 epitope of human mid-frontal gyrus brain tissue in immunohistochemistry. Clone ID McKA1 Isotype IgG2a Preservative None Format Purified with protein G, stored in PBS pH 7.4 and lyophilized. Recommend starting dilution If reconstituted with deionized water in 100 µL: ICC / IHC 1:5,000. Optimal dilution has to be determined by the user Limitations Research Use Only Storage Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.