Target TC-PTP (N term) Target background The protein tyrosine phosphatases (PTP) belongs to a large family of enzymes involved in the regulation of cellular signaling and homeostasis, each possessing a 240 amino acid PTP domain. The T-cell protein tyrosine phosphatase (TC-PTP) is predominantly expressed in the hematopoietic lineage. TC-PTP null mice suffer severe defects in the hematopoietic compartment and die at 3-5 weeks of age from severe anemia. TC-PTP -/- murine embryonic fibroblasts have a slow progression through the G1 phase of the cell cycle and have compromised NF-kappaB activation. JAK 1 and 3 have been identified as physiological substrates of TC-PTP. Recent genetic studies have revealed an association between the PTPN2 (TC-PTP) gene and diseases like type 1 diabetes and Crohn’s disease. Target alias T-cell protein tyrosine phosphatase, Protein-tyrosine phosphatase PTP-2, MPTP, Ptpn2, Ptpt Immunogen N-terminal fragment of TC-PTP Specificity The antibody recognizes the N-terminal portion of TC-PTP; when used for Western blots the antibody detects one band in MEF cells extract, and 2 bands in spleen extract. The antibody recognizes 4 bands of TC-PTP from monkey Clone ID 3e+02 Isotype IgG1 Preservative None Format Purified with protein G, stored in PBS pH 7.4 and lyophilized. Recommend starting dilution If reconstituted with deionized water in 100 µL: WB 1:1000. Optimal dilution has to be determined by the user. Limitations Research Use Only Storage Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay. References