Target Horseradish Peroxidase (HRP) Target background Horseradish peroxidase (HRP) is a 40 kilodalton (kDa) glycoprotein with four lysine residues. In the presence of hydrogen peroxide, HRP will oxidize a substrate to yield a detectable signal useful for techniques such as ELISA and Immunohistochemistry. A monoclonal rat anti-peroxidase antibody is a very useful and reliable reagent in the localization of a number of rat primary antibodies by peroxidase anti-peroxidase (PAP) immunocytochemistry. Target alias HRP Immunogen HRP protein Specificity The antibody recognizes Horseradish peroxidase (HRP). Clone ID YP4 Isotype IgG1 Preservative None Format Purified with protein G, stored in PBS pH 7.4 and lyophilized. Recommend starting dilution If reconstituted with deionized water in 100 µL: IHC: 1:50 – 1:200. Optimal dilution has to be determined by the user. Limitations Research Use Only Storage Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.