Target Huntingtin-Interacting Protein 1-Related (HIP1R) Target background HIP1R (huntingtin interacting protein 1 related) was first cloned based on its sequence similarity with HIP1 (Huntingtin Interacting Protein 1). HIP1 binds to normal huntingtin but this interaction is lost in Huntington’s disease (HD). HIP1 also binds to components of clathrin-coated pits and vesicles and is a marker of clathrin-coated membranes. HIP1R colocalizes with and binds to actin and this interaction may link the endocytic machinery to the actin cytoskeletal dynamic processes. Target alias HIP1-related protein, Huntingtin-interacting protein 12, HIP-12, HIP1R Immunogen peptide in C term Specificity The antibody recognizes the C-terminal portion of HIP1R Clone ID --- Preservative None Format Immunogen affinity purified in glycerol/PBS pH7.4 Recommend starting dilution If diluted with deionized water in 100 µL: WB 1:500-1:2500; IF 1:50-1:250. Optimal dilution has to be determined by the user. Limitations Research Use Only Storage Antibodies diluted in glycerol can be kept at 4ºC for up to 2 months and should be kept at -20ºC for long-term storage (1 year). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. After the maximum long-term storage period (1 year -20ºC or 2 months 4ºC) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.