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Target
Staufen2
Target background
Staufen, RNA binding protein homolog 2, is a member of the family of double-stranded RNA-binding proteins. Staufen 2 is mainly expressed in brain and heart tissues. Staufen 2 is found in the somatodendritic domain of neurons and colocalizes with microtubules in dendrites. The exact function of Staufen 2 is not yet known, but it could be involved in events involving double-stranded RNA-binding
Target alias
Double-stranded RNA-binding protein Staufen homolog 2, STAU2
Immunogen
peptide in C term
Specificity
The antibody recognizes the C-terminal of the human Staufen 2 protein
Clone ID
1C6
Isotype
IgG
Preservative
None
Format
Purified with protein G, stored in PBS pH 7.4 and lyophilized.
Recommend starting dilution
If reconstituted with deionized water in 250 µL: IF 1:250; WB 1:550. Optimal dilution has to be determined by the user.
Limitations
Research Use Only
Storage
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.