Target Tyrosine Hydroxylase Target background Tyrosine hydroxylase is the rate-limiting enzyme in the catecholamine synthesis. It is responsible for the hydroxylation of L-tyrosine, which is further converted to give rise to dopamine, norepinephrine and epinephrine. Target alias Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH, TOH Immunogen Full length TOH Specificity The antibody recognizes the full length TOH Clone ID TOH A1.1 Isotype IgG1 Preservative None Format Purified with protein G, stored in PBS pH 7.4 and lyophilized. Recommend starting dilution If reconstituted with deionized water in 500 µL: IHC 1:100 - 1:250. Optimal dilution has to be determined by the user. Limitations Research Use Only Storage Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.