Target Collagen XVII Target background Collagen XVII is a member of the transmembrane collagen family of proteins. It is a major structural component of the hemidesmosome (HD) playing an important role in its integrity. HD is a specialized multiprotein complex involved in the attachment of basal keratinocytes to the underlying basement membrane. Target alias Collagen alpha-1(XVII) chain, 180 kDa bullous pemphigoid antigen 2, Bullous pemphigoid antigen 2, COL17A1, BP180, BPAG2 Immunogen Purified recombinant NC16a-domain of collagen XVII Specificity The antibody recognizes the NC16a domain (aa545-557) Clone ID NC16a-3 Isotype IgG1 Preservative None Format Purified with protein G, stored in PBS pH 7.4 and lyophilized. Recommend starting dilution If reconstituted with deionized water in 250 µL: WB 1:1000; IHC-P: 1:40. Optimal dilution has to be determined by the user Limitations Research Use Only Storage Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.