Target ADP ribosylation factor 6 (Arf6) Target background The ARF subfamily comprises a group of proteins that are members of the Ras superfamily of small guanosine triphosphatases (small GTPases). Six ARFs have been identified by related gene products from different species. These 6 members can be divided into three classes on the basis of their size and amino acid sequence homology. ARF6 is the only member of the Class III. ARF6 has a variety of cellular functions that are frequently involved in trafficking of biological membranes and transmembrane protein cargo and has specifically been implicated in endocytosis of plasma membrane proteins and plasma membrane protein recycling. Specificity The antibody recognizes the human human ADP ribosylation factor 6 (Arf6) protein. Clone ID --- Preservative None Format Lyophilized serum Recommend starting dilution If reconstituted with deionized water in 100 µL: WB: 1:2000; IHC: 1:100-1:250. Optimal dilution has to be determined by the user. Limitations Research Use Only Storage Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.