Target ARNO (Cytohesin-2) Target background ARNO / Cytohesin-2 acts as guanine-nucleotide exchange factor (GEF) for ADP-ribosylation factor such as ARF1, ARF3 and ARF6. Both isoforms (1 and 2) of ARNO / Cytohesin-2 are located in the cytoplasm and in cell membrane after being recruited through their association with ARL4A, ARL4C and ARL4D Specificity A specific antibody against the human ARNO / Cytohesin-2 protein. Clone ID --- Preservative None Format Lyophilized serum Recommend starting dilution If reconstituted with deionized water in 100 µL: WB: 1:2000; IHC: 1:100-1:250. Optimal dilution has to be determined by the user. Limitations Research Use Only Storage Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.