Target Rab27a Target background Ras-related protein Rab-27a (Rab27a) belongs to the small GTPase superfamily. The protein is membrane-bound and is involved in protein transport and small GTPase-mediated signal transduction. Rab27a plays a role in cytotoxic granule exocytosis in lymphocytes. Rab27a is widely expressed with considerable expression in pancreatic islets and pituitary tissue, and low expression in the brain. Defects in RAB27A are a cause of Griscelli syndrome type 2 (GS2), a rare autosomal recessive disorder. Target alias Ras-related protein Rab-27A, RAB27A, GS2, HsT18676, RAB27, RAM, member RAS oncogene family Specificity The antibody recognizes the human Rab27a protein. Clone ID --- Preservative None Format Lyophilized serum Recommend starting dilution If reconstituted with deionized water in 100 μl: WB: 1:1000 (Recommended blocking solution: Donkey Serum 20%). IP: native conditions with 0.2% TritonX-100. Optimal dilution has to be determined by the user. Limitations Research Use Only Storage Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.