Target RhoGDI alpha Target background Rho GDP-dissociation inhibitor 1 (RhoGDI alpha) belongs to the Rho GDI family of proteins. It is found in the cytoplasm where it regulates the GDP/GTP exchange reaction of the Rho, Rac and Cdc42 proteins by inhibiting their dissociation from GDP, and their subsequent binding to GTP. Rho, Rac and Cdc42 are molecular switches involved in the regulation of cytoskeletal structures that affect the regulation of cell morphology, movement, attachment, phagocytosis and cytokinesis. Target alias Rho GDP-dissociation inhibitor 1, RhoGDI alpha Specificity The antibody recognizes the human RhoGDI alpha protein. Clone ID --- Preservative None Format Lyophilized serum Recommend starting dilution If reconstituted with deionized water in 100 µl: WB 1:1000 – 1:2000. Optimal dilution has to be determined by the user. Limitations Research Use Only Storage Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.