Target VAT1 (N-terminal) Target background Vesicle Amine Transport protein-1 (VAT1) plays a role in calcium-regulated keratinocyte activation in epidermal repair mechanisms through its ATPase activity. It is expressed in the brain, glioblastoma cells and expression has been detected following wounding. The expression of VAT1 is calcium ion-dependent. Target alias VAT1, VATI, vesicle amine transport 2 Specificity The antibody recognizes the N-terminal of the human Rab27a protein (aa1-16). Clone ID --- Preservative None Format Lyophilized serum Recommend starting dilution If reconstituted with deionized water in 100 µl: WB: 1:1000. Optimal dilution has to be determined by the user. Limitations Research Use Only Storage Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay. References