Target SMAP2 (WB) Target background Stromal membrane associated protein-2 (SMAP2, also known as small ArfGAP2) is a GTPase activating protein that plays a role in clathrin-dependent endocytosis. SMAP2 is known to interact with ARF1 and ARF6 (in vitro) and may play a role in clathrin-dependent retrograde transport from early endosomes to the trans-Golgi. It is expressed in multiple foci throughout the cytoplasm and in juxtanuclear structures. Target alias Small ArfGAP2, stromal membrane associated protein-2 Specificity The antibody recognizes the human small ArfGAP2 protein (aa 127-251). Clone ID --- Preservative None Format Lyophilized serum Recommend starting dilution If reconstituted with deionized water in 100 µl: WB 1:1000. Optimal dilution has to be determined by the user. Limitations Research Use Only Storage Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.