Target Nucleobindin Target background Nucleobindin 1 is a 461 amino acids ubiquitous protein with an N-terminal of 25 amino acids signal peptide responsible for its initial localization to the endoplasmic reticulum (ER). It has also been detected in the cytoplasm and in various subcellular structures such as the nucleus, mitochondria and the Golgi apparatus. Nucleobindin plays a role in a number of cellullar processes such as intracellular signaling, inflammation, autoimmunity, osteogenesis and cancer. Target alias NUCB1, NUC, CALNUC Immunogen Full length recombinant human nucleobindin protein. Specificity The antibody recognizes nucleobindin 1 (NucB1) Clone ID --- Preservative None Format Immunogen affinity purified, stored in PBS pH7.4 and lyophilized. Recommend starting dilution If reconstituted with deionized water in 100 µg: WB: 1:1000; IF: 1:150; EM-ICC: 1:50. Optimal dilution has to be determined by the user. Limitations Research Use Only Storage Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.