Target Chromogranin-A (N-term) Target background Chromogranin-A is a secreted protein known as a “prohormone” which is found in endocrine cells and neurons and may be released upon stimulation. It is thought to be involved in the formation of dense core granules, where it may be processed to form the bioactive peptide serpinin, which is thought to regulate the biogenesis of dense core granules in endocrine cells. Chromogranin-A is used as a biomarker in patients with neuroendocrine tumors. Recent publications show that incomplete processing of Chromogranin-A may be linked to diabetic retinopathy Target alias hCGA, parathyroid secretory protein 1, CHGA Immunogen N-terminal peptide (AA 4-16) Specificity The antibody recognizes an epitope in N-terminal (AA 4-16) of Chromogranine-A Clone ID --- Preservative None Format Lyophilized serum Recommend starting dilution If reconstituted with deionized water in 100 µl: WB 1:2000. Optimal dilution has to be determined by the user. Limitations Research Use Only Storage Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay. References