Target Cdc5 Target background Polo-like kinases (PLKs) are evolutionarily conserved kinases essential for cell division, proliferation, and development. These kinases are characterized by the presence of a C-terminal phosphor-peptide-interaction domain, the polo-box domain (PBD). Cdc5 (Msd2) is a cell cycle serine/threonine-protein kinase of Saccharomyces cerevisiae involved in mitotic exit. Recently, a novel function for Cdc5 as an important regulator of cellular ploidy has been reported. Target alias Cell cycle serine/threonine-protein kinase CDC5/MSD2, MSD2, PKX2 Immunogen Full length protein Specificity The antibody recognizes the full length protein Cdc5 (Msd2) Clone ID 4F10 Isotype IgG1 Preservative None Format Purified with protein G, stored in PBS pH 7.4 and lyophilized. Recommend starting dilution If reconstituted with deionized water in 100 µl: WB 1:250-1:500. Optimal dilution has to be determined by the user. Limitations Research Use Only Storage Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.