Target PTEN Target background Phosphatase and tensin homolog on chromosome ten (PTEN) is a ubiquitously expressed tumor suppressor which, in the cytoplasm, antagonizes the PI3K signaling pathway through its lipid phosphatase activity. Mutations of this gene are an important step in the development of many cancers since PTEN regulates essential cellular process such as growth and survival. PTEN is also found in the nucleus, and studies have demonstrated that its presence in the nucleus is regulated by SUMOylation and phosphorylation. Also, studies have showed that cells lacking nuclear PTEN are more sensitive to DNA damage. Target alias Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase Immunogen C-terminal peptide Specificity The antibody recognizes the C-terminal portion of PTEN. Clone ID 6H2.1 Isotype IgG2a kappa Preservative None Format Purified with protein G, stored in PBS pH 7.4 and lyophilized. Recommend starting dilution If reconstituted with deionized water in 100 µl: WB 1:1000, IP 1:100, IHC 1:200. Optimal dilution has to be determined by the user. Limitations Research Use Only Storage Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay. References