Target Pseudomonas aeruginosa Outer Core Target background Pseudomonas aeruginosa is a Gram-negative bacterium widely distributed in nature and causing opportunistic infections in humans. P. aeruginosa is an important bacterial pathogen of nosocomial (hospital derived) infections, and it can also cause life threatening diseases in patients with cancer, burn wounds, cystic fibrosis and those that have received immunosuppressive therapy. Lipopolysaccharide (LPS) is an integral component of the P. aeruginosa cell envelope, occupying the outer leaflet of the outer membrane in this pathogen. The outer core of P. aeruginosa LPS is composed of one d-galactosamine (GalN), one l-Rha, and three or four d-glucose (GlcI–GlcIV) residues. It exists in two structurally distinct glycoforms, called “uncapped” and “capped” which basically differ in position and linkage of an l-Rha residue in each structure Target alias P. aeruginosa Outer Core Specificity The antibody recognizes the Outer-core OS of P. aeruginosa LPS that has terminal D-glucose residue, as observed in strain PAO1 (serotype O5), but not in strain serotype O6. Clone ID 5c-101 Isotype IgG3 Preservative None Format Lyophilized protein free tissue culture supernatant Recommend starting dilution If reconstituted with deionized water in 100 µl: E: 1:1,000. Optimal dilution has to be determined by the user. Limitations Research Use Only Storage Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.