Target TRBP Target background TRBP or HIV-1 TAR RNA binding protein is a dsRBP with two dsRNA binding domains (dsRBDs). The recruitment of Ago2 to the siRNA bound by Dicer requires the action of TRBP. TRBP increases viral mRNA translation and inhibit interferon-induced PKR which results in increase of HIV-1 expression and replication. The RNA-induced silencing complex (RISC) is partly composed by Dicer, Ago2, and TRBP. TRBP2 has an extra 21AA in N-term compared to TRBP1. Target alias HIV-1 TAR RNA binding protein, TRBP1, TRBP2 Immunogen Synthetic peptide Specificity The antibody recognizes the TRBP1 and TRBP2 protein Clone ID 4A6 Isotype IgG1 kappa Preservative None Format Purified with protein G, stored in PBS pH 7.4 and lyophilized. Recommend starting dilution Reconstitute with deionized water. Optimal dilution has to be determined by the user. Limitations Research Use Only Storage Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.