Target SWE1 Target background Homologue of Wee1, Swe1 is a checkpoint kinase found in the budding yeast Saccharomyces cerevisiae. Swe1 imposes a delay between the G2 and M phases of the cell cycle, inhibiting entry into mitosis by inhibiting the activity of Cdc28 through phosphorylation at tyrosine residue 19. Swe-1 is negatively regulated by the proteins Hsl1 and Hsl7, and a complex of the three proteins is localized at the bud neck of the yeast. Swe1 is also thought to be SUMOylated, and ubiquitination of Swe1 controlled by Dma1 and Dma2 ubiquitin ligases. The kinase Swe1 controls mitotic spindle elongation and is thought to regulate the filamentous growth and differentiation of S. cerevisiae. Target alias Mitosis inhibitor protein kinase SWE1, Wee1 homolog Immunogen Recombinant Swe1 protein Specificity The antibody recognizes the Swe1 protein in yeast synchronized in S phase, and is negative for yeast synchronized in G1 phase. Clone ID 18D9G8A1 Isotype IgG2a kappa Preservative None Format Purified with protein G, stored in PBS pH 7.4 and lyophilized. Recommend starting dilution If reconstituted with deionized water in 100 µl: WB: 1:250. For western blots, it is strongly recommended that MM-0273 anti-Swe1 (18D9G8A1) and MM-0274 anti-Swe1 (15F2) be used in combination at a 1:250 dilution for efficient detection of Swe1. Optimal dilution has to be determined by the user. Limitations Research Use Only Storage Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay. References