Target PSMA Target background The prostate-specific membrane antigen (PSMA), also known as Glutamate carboxypeptidase II (GCPII) or N-acetyl –L-aspartyl-L-glutamate peptidase I (NAALDAse I), is an integral type II cell-surface zinc-metalloprotease of 750 amino acids. PSMA is predominantly expressed in epithelial cells of prostate tissue, and also found in the brain, kidney tubules, small bowel, and in endothelial cells in certain solid tumor neovasculature. Its expression in normal and malignant prostate correlates with tumor aggressiveness, with highest levels in hormone refractory and metastatic disease. Target alias Glutamate carboxypeptidase II (GCPII), N-acetyl –L-aspartyl-L-glutamate peptidase I (NAALDAse I) Immunogen Synthetic peptide of human PSMA comprised of amino acids 490-500. Specificity The antibody recognizes the extracellular residues 490-500 of human PSMA. The anitbody is ot reactive to mouse or rat PSMA homologue. Clone ID 17G1 Isotype IgG1 kappa Preservative None Format Purified with protein G, stored in PBS pH 7.4 and lyophilized. Recommend starting dilution Reconstitute with deionized water. Optimal dilution has to be determined by the user. Limitations Research Use Only Storage Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.