Target Somatostatin Target background Somatostatin or growth hormone-inhibiting hormone (GHIH) is a peptide hormone that inhibits the release of somatotropin (growth hormone) from the anterior pituitary gland in the hypothalamus. Somatostatin also inhibits the release of peptide hormones in many other tissues by binding to high-affinity G protein-coupled somatostatin receptors. Two active forms have been described: a form that is 14 amino acids in length and a form that is 28 amino acids in length, which are produced by alternate cleavage of the single pre-pro-protein encoded by this gene. Target alias Growth hormone–inhibiting hormone (GHIH), Growth hormone release–inhibiting hormone (GHRIH), Somatotropin release–inhibiting factor (SRIF), Somatotropin release–inhibiting hormone (SRIH) Immunogen Synthetic peptide Specificity The antibody recognizes somatostatin. The antibody shows no cross-reactivity with any of the following substances: insulin, glucagon, secretin, gastrin, TRH, LHRH, MIF, neurophysin, oxytocin, vasopressin, substance P, GIP, rGH, rLH, rTSH, and rPRL. Clone ID --- Preservative None Format Lyophilized serum Recommend starting dilution If reconstituted with deionized water in 50 µl: In vivo Passive Immunization: neat serum, RIA: in a RIA for SRIF, at a final dilution of 1: 25,000, this antiserum binds 40% of [125I]-tyri-SRIF and can detect 10pg/assay tube. Optimal dilution has to be determined by the user. Limitations Research Use Only Storage Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.