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BeyoAb™ ERK1/2 Rabbit Polyclonal Antibody
  • 商城价格 登录后可查看价格
  • 货号 AB2014-50μl
  • 品牌 Beyotime/碧云天 ( 生产厂家 )
  • CAS号 见包装
  • 规格/包装 50μl
  • 单位 件
  • 储存条件 -20℃
  • 现货状态 三个工作日
  • 数量

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来源 用途 交叉反应性 理论分子量 实际分子量
Rabbit WB, IF, IHC, ICC H, M, Rb ERK1: 44KDa
ERK2: 42KDa 		ERK1: 44KDa
ERK2: 42KDa

WB, Western blot; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry. H, Human; M, Mouse; Rb, Rabbit.

BeyoAb™系列抗体为经深入测试的优质抗体，并配套提供Western一抗稀释液，可用于Western或其它适当用途的一抗稀释。

建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整，NT, not tested)。

WB IP IF IHC ICC FC ChIP
1:1000-1:2000 NT 1:50-1:200 1:50 1:50-1:200 NT NT

本抗体相关应用的代表性检测效果图片请参见Fig. 1。

Fig. 1. BeyoAb™ ERK1/2 Rabbit Polyclonal Antibody (AB2014) was used for Western blot, immunofluorescence and immunohistochemistry.

A. Western analysis of lysates from indicated cell lines using BeyoAb™ ERK1/2 Rabbit Polyclonal Antibody (AB2014) at 1:1000 dilution and BeyoWB™ HRP-labeled Goat Anti-Rabbit IgG (H+L) (P0948) at 1:500 dilution. 15µg protein per lane was loaded and analyzed by BeyoWB™ 80min Electrophoresis, Transfer and Western Blot Kit (P0002). Exposure time, 2s.

B. Immunofluorescence analysis of HeLa cells using BeyoAb™ ERK1/2 Rabbit Polyclonal Antibody (AB2014) at a dilution of 1:100 and Cy3-labeled Goat Anti-Rabbit IgG (H+L) (A0516) at 1:250 dilution. a. Brightfield. b. Immunofluorescence image for ERK1/2. c. Nuclear staining using Antifade Mounting Medium with DAPI (P0131). d, Merged image for b and c.

C. Immunohistochemistry of a section from paraffin-embedded mouse stomach tissue using BeyoAb™ ERK1/2 Rabbit Polyclonal Antibody (AB2014) at a dilution of 1:50 (bottom panel) compared to a negative control without primary antibody (Top panel). HRP-labeled Goat Anti-Rabbit IgG (H+L) (A0208) was used as secondary antibody at 1:1000 dilution.

抗体详细信息如下:

About this Antibody
Name BeyoAb™ ERK1/2 Rabbit Polyclonal Antibody
Category Polyclonal antibody (pAb); Primary antibody
Isotype IgG
Purification method Protein A affinity purification
Positive samples HeLa; NIH/3T3
Cellular location -
Customer validation -
About the Immunogen
Immunogen Recombinant protein
Sequence -
Gene ID ERK1: 5595; ERK2: 5594
SwissProt ERK1: P27361; ERK2: P28482
Synonyms ERK; p38; p40; p41; ERT1; MAPK2; PRKM1; P42MAPK; p41mapk; MAPK1; ERK1; ERK2; Mitogen-activated protein kinase 1; Mitogen-activated; protein kinase 2
Category MAPK
Background Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G protein-coupled receptor ligands and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK 1 and ERK 2 requires phosphorylation by upstream kinases such as MAP kinasekinase (MEK), MEK kinase and Raf-1 [1]. ERK 1 and ERK 2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the threonine-glutamate-tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development. The human ERK 1 gene maps to chromosome 16p11.2 and encodes a 379 amino acid protein that shares 83% sequence identity to ERK 2. The human ERK2 gene maps to chromosome 22q11.21 and encodes a 360-amino acid protein [2].
包装清单：
产品编号 产品名称 包装
AB2014-50μl BeyoAb™ ERK1/2 Rabbit Polyclonal Antibody 50μl
AZ050 Western一抗稀释液 50ml
— 说明书 1份

产品编号 产品名称 包装
AB2014-200μl BeyoAb™ ERK1/2 Rabbit Polyclonal Antibody 200μl
AZ200 Western一抗稀释液 200ml
— 说明书 1份
保存条件：

-20ºC保存，至少一年有效。Western一抗稀释液也可以4ºC保存，一年有效。Western一抗稀释液优先推荐4ºC保存，长期不使用可以考虑-20ºC保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。

注意事项：

如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4ºC保存。

回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000×g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。

提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。

本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。

为了您的安全和健康，请穿实验服并戴一次性手套操作。

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