官网https://www.novoprotein.com.cn/product-detail?productNumber=C083
产品描述
Recombinant Human Vascular Endothelial Growth Factor A is produced by our Mammalian expression system and the target gene encoding Ala27-Arg191 is expressed.
蛋白编号
P15692-4
产品别称
Vascular Endothelial Growth Factor Isoform 165; VEGF165
偶联
Unconjugated
分子量
19.1 KDa
表观分子量
18-24 KDa, reducing conditions
剂型描述
Lyophilized from a 0.2 μm filtered solution of 20mM Citrate, 8% Sucrose, 4% Mannitol, 0.05% Tween 80, pH4.0.
内毒素
< 0.01 EU/µg as determined by LAL test.
纯度-SDS-PAGE

Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
生物活性-ELISA

Immobilized Recombinant Human VEGF165(C083) at 2μg/ml (100 μl/well) can bind Human VEGF-A Antibody (Bevacizumab)(Human IgG1)(NC216). The ED50 of Human VEGF-A Antibody (Bevacizumab)(Human IgG1)(NC216) is 2.20 ng/ml. (Regularly tested)

Immobilized Human VEGF165 (C083) at 10μg/ml (100 μl/well) can bind Human VEGFR1-Fc (CJ93). The ED50 of Recombinant Human VEGFR1-Fc (CJ93) is 36.4 ng/ml. (Regularly tested)
生物活性-BLI

Loaded Brolucizumab on AMC Biosensor, can bind Recombinant Human VEGF165 (C083) with an affinity constant of 22.2 pM as determined in BLI assay. (Regularly tested)

Loaded Human VEGF-A Antibody (Bevacizumab) (NC216) on Pro-A Biosensor, can bind Recombinant Human VEGF165 (C083) with an affinity constant of 7.12 pM as determined in BLI assay. (Regularly tested)

Loaded Human VEGFR1-Fc(CJ93) on Protein A Biosensor, can bind Human VEGF165(C083) with an affinity constant of 0.29 nM as determined in BLI assay. (Regularly tested)
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.