商品介绍

gene_symbol：Sonic Hedgehog
Synonyms：HHG1; HLP3; HPE3; MCOPCB5; SMMCI; TPT; TPTPS
clone_name：OTI10H6
immunogen：Human recombinant protein fragment corresponding to amino acids 24-197 of human SHH (NP_000184) produced in E.coli.
predicted_size：27.6 kDa
background：SHH is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo.
buffer：PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
purification：Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
isotype：IgG1
host：Mouse
applications：IF, WB
Recommend Dilution：WB 1:1000~2000, IF 1:100
reactivities：Human, Dog, Rat, Monkey, Mouse
storage：Store at -20°C as received.