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来源 | Isotype | 用途 | 交叉反应性 | 标记 | Ex/Em |
Mouse | IgG1 | FC | Human | PE | 565/578nm |
注:推荐用于流式细胞仪检测每次用量为2.5µl,对应2.5-5×105细胞或50µl外周血,实际使用时可以根据样品的实际情况适当调整抗体用量。
碧云天的BeyoFC™系列抗体,也称BeyoFC™流式抗体(Antibody for flow cytometry, FC antibody),是经过充分验证适用于流式细胞仪(Flow Cytometry, FC)的高品质抗体,BeyoFC™系列流式抗体提供很多常见抗体,并提供多种荧光标记,便于选择合适的抗体和适当的荧光标记。
BeyoFC™系列流式抗体对于细胞的标记效果好,染色背景低,细胞分群更清晰。
BeyoFC™系列流式抗体特异性高,染色和细胞分群结果更为准确。
BeyoFC™系列流式抗体一致性好,批次间差异更小,结果重复性好。
抗体详细信息如下:
About this Antibody | |
Name | BeyoFC™ AC0663 Antibody |
Category | Mouse Monoclonal Antibody (mAb); Primary antibody; FC Antibody |
Isotype | IgG1 |
Clone number | BY143-13 |
Purification method | Protein A affinity purification |
About the Immunogen | |
Gene ID | 3559 |
Swiss Prot | P01589 |
Synonyms | p55; CD25; IL2R; IMD41; TCGFR; IDDM10 |
Background | The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. Patients with severe Coronavirus Disease 2019 (COVID-19), the disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have significantly elevated levels of IL2R in their plasma. Similarly, serum IL-2R levels are found to be elevated in patients with different types of carcinomas. Certain IL2RA and IL2RB gene polymorphisms have been associated with lung cancer risk. [provided by RefSeq, Jul 2020] |
产品编号 | 产品名称 | 包装 |
AC0663-125μl | BeyoFC™ PE anti-human CD25 Antibody | 125μl |
AFC060 | BeyoFC™流式检测细胞染色缓冲液 | 60ml |
— | 说明书 | 1份 |
产品编号 | 产品名称 | 包装 |
AC0663-500μl | BeyoFC™ PE anti-human CD25 Antibody | 500μl |
AFC250 | BeyoFC™流式检测细胞染色缓冲液 | 250ml |
— | 说明书 | 1份 |
4ºC保存,一年有效。抗体须避光保存。BeyoFC™流式检测细胞染色缓冲液-20ºC可以保存更长时间。
注意事项:流式抗体通常不宜回收再使用。
相关步骤离心时,4ºC可更好的维持细胞完整性;同时根据细胞类型,可对离心力进行一定的优化。
如果需要同时进行细胞表面和细胞内抗原的流式检测,须先进行细胞表面抗原的染色,这样可以避免有些细胞表面抗原因为固定而无法被流式抗体所识别。
如需更多的BeyoFC™流式检测细胞染色缓冲液,可以另行单独采购BeyoFC™流式检测细胞染色缓冲液(C1711)。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。