Description The ImmunoStar VMAT2 antiserum was quality control tested using standard immunohistochemical methods in rat brain and adrenal medulla using biotin/avidin-HRP techniques. Specificity of the antiserum was demonstrated by soluble pre-adsorption and Western blot. Tissue staining is completely eliminated by pretreatment of the diluted antibody with an excess of rat VMAT2 peptide residues 496-515. Western blot analysis of immunoprecipitated rat brain homogenates demonstrates a dense immunoreactive band of approximately 55 kD and a minor band of approximately 75 kD. Photo Description: IHC image of VMAT2 staining in the rat adrenal medulla (above) and of neurons in the pontine region of the rat brain (below). The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning for rat brain and frozen sectioning for adrenal gland. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. Host: Rabbit Quantity / Volume: 100 µL State: Lyophilized Whole Serum Species Reactivity: Rat Availability: In Stock Alternate Names: Monoamine transporter; Solute carrier family 18 member 2; Synaptic vesicular amine transporter; Vesicular amine transporter 2; Solute carrier family 18 A2 (vesicular monoamine transporter 2); SLC18A2; SVMT; VAT2; Svat; mnat, anti-VMAT2 Gene Symbol: Slc18a2 RRID: AB_10013884 Database Links: Entrez Gene: 25549 Rat