Description The antibody has a proven strong biotin-streptavidin/HRP staining at a 1/1000 – 1/2000 dilution in rat globus pallidus and spinal cord. Staining is completely eliminated by pretreatment with 50 µg of Leucine Enkephalin per mL of diluted antiserum. Pretreatment with 50 µg of Methionine Enkephalin per mL of diluted antiserum also significantly blocks staining. Photo Description: Low magnification IHC image of leucine enkephalin staining in the rat globus pallidus. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. Host: Rabbit Quantity / Volume: 100 µL State: Lyophilized Whole Serum Reacts With: Bird, Bovine, Bullfrog, Cat, Chick, Dog, Fish, Frog, Guinea Pig, Hamster, Human, Lungfish, Monkey, Mouse, Octopus, Pig, Pigeon, Rat, Ray, Shark, Sheep, Starling, Three-Spined Stickleback, Turtle, Zebra Finch Availability: In Stock Alternate Names: Enkephalin (Leu+Met); Proenkephalin-A; enkephalin A; preproenkephalin, anti-Leucine Enkephaline, Leu Enk Gene Symbol: PENK