Description The antibody has a proven strong Biotin-Streptavidin/HRP staining at a 1/1000-1/2000 dilution in rat hypothalamus (median eminence). The specificity of the antiserum was examined by soluble pre-adsorption with the peptides in question at a final concentration of 106M. Somatostatin immunolabeling was completely abolished by pre-adsorption with somatostatin, somatostatin 25, and somatostatin 28. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: substance P, amylin, glucagon, insulin, neuropeptide Y, and VIP. Photo Description: IHC image of the median eminence staining for somatostatin (above). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. Florescent secondary (below). Host: Rabbit Quantity / Volume: 100 µL State: Lyophilized Whole Serum Reacts With: Alligator, Anuran (Frog), Bat, Bird, Buffalo (Bubalus Bubalis), Bullfrog, Cat, Chick, Chicken, Dermophis Mexicanus Mexican Caecilian (Amphibian ), Dog, Duck, Fish, Fowl, Frog (Xenopus Laevis), Guinea Pig, Hamster, Human, Lizard, Malayan Pangolin (Sunda Pangolin), Manducta Sexta (Moth), Monkey, Mouse, Pig, Pigeon, Quail, Rabbit, Raccoon, Rana Pipiens (Leopard Frog), Rat, Scorpaena Porcus (Fish), Sea Bass (Fish), Sheep, Snail, Snake, Sparus Aurata (Fish), Starfish, Sting Ray, Turtle, Zebrafish Availability: In Stock Preabsorption Control: Available at Sigma Aldrich, Catalog # S9129 Alternate Names: Growth hormone release-inhibiting factor; Somatostatin 14; Somatostatin 28; prepro-somatostatin; SMST; anti-somatostatin Gene Symbol: SST