Description The antibody produces a maximum biotin-streptavidin/HRP staining at a 1/500 – 1/1000 dilution in rat anterior/intermediate pituitary. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. The recommened dilution series is 1/500-1/1000 in PBS/0.3% Triton X-100 – Bn-SA/HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 100 µg/mL of ACTH. Photo Description: IHC image of neurons staining for ACTH in the rat anterior pituitary gland. The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:1000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. Host: Rabbit Quantity / Volume: 100 µL State: Lyophilized Whole Serum Availability: In Stock Reacts With: Dove, Eel, Goldfish, Hamster, Human, Monkey, Mouse, Ram (Sheep), Rat, Trout Alternate Names: anti-ACTH RRID: AB_572216 Immunogen: Adrenocorticotropic Hormone Gene Symbol: POMC Database Links: Entrez Gene: 102092785 Dove Entrez Gene: 100756311 Hamster Entrez Gene: 5443 Human Entrez Gene: 694430 Monkey Entrez Gene: 18976 Mouse Entrez Gene: 443212 Ram (Sheep) Entrez Gene: 24664 Rat Entrez Gene: 100136771 Trout NCBI Gene Aliases: ACTHR,MGC12579