Description The ImmunoStar Neurotensin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat amygdala using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/4000-1/8000 in PBS/0.3% Triton x-100 – Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment with 10 µg of Neurotensin per 1 mL of diluted antibody. Photo Description: IHC image of rat amygdala staining for neurotensin. The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. Host: Rabbit Quantity / Volume: 100 µL State: Lyophilized Whole Serum Reacts With: Cat, Dog, Frog, Human, Monkey, Mouse, Pigeon, Rat, Turtle Availability: In Stock Alternate Names: neuromedin N; Preproneurotensin; pro-neurotensin, anti-neurotensin Gene Symbol: NTS RRID: AB_572254 Database Links: Entrez Gene: 101095835 Cat Entrez Gene: 611687 Dog Entrez Gene: 100485937 Frog Entrez Gene: 4922 Human Entrez Gene: 700189 Monkey Entrez Gene: 67405 Mouse Entrez Gene: 102087679 Pigeon Entrez Gene: 299757 Rat Entrez Gene:102446489 Turtle