Description The ImmunoStar Gastrin Releasing Peptide antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat dorsal horn of spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/1,000-1/2,000 in PBS/0.3% Triton X-100 – Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment of 1 mL of diluted antibody with 50 µg of bombesin. Photo Description: IHC of at stomach staining for gastrin releasing peptide. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen and counterstained with hematoxylin Host: Rabbit Quantity / Volume: 100 µL State: Lyophilized Whole Serum Reacts With: Chick, Hamster, Human, Mouse, Newt, Pigeon, Quail, Rabbit, Ram (Sheep), Rat, Zacco Platypus (Minnow) Availability: In Stock Preabsorption Control: Available at irbiologicals, Catalog # IRCP069. Sequence: Glp-Gln-Arg-Leu-Gly-Asn-Gln-Trp-Ala-Val-Gly-His-Leu-Met-NH Alternate Names: BN; GRP-10; proGRP; preproGRP; gastrin-releasing peptide, anti-GRP, Bombesin, anti-Bombesin Gene Symbo: GRP RRID: AB_572221 Database Links: Entrez Gene: 425213 Chick Entrez Gene: 100769410 Hamster Entrez Gene: 2922 Human Entrez Gene: 225642 Mouse Entrez Gene: 102093899 Pigeon Entrez Gene: 107325907 Quail Entrez Gene: 103349406 Rabbit Entrez Gene: 443332 Ram (Sheep) Entrez Gene: 171101 Rat