Description The antibody produces a strong postive labeling of LHRH / GnRH at dilution of 1/2,000 – 1,4,000 using biotin-streptavidin/HRP in rat hypothalamus (median eminence). Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. The above image is of LHRH (GnRH) terminals in rat median eminence, ryostat sections, ABC / DAB detection. Staining is completely eliminated by pretreatment of the diluted antibody with 5 µg of LHRH per mL of diluted antiserum. Photo Description: IHC image of staining for LNRH/GnRH in the rat median eminence. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:4000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. Host: Rabbit Quantity / Volume: 100 µL State: Lyophilized Whole Serum Reacts With: Bat, Carp, Catfish, Chick, Chicken, Clarias Batrachus (Catfish), Fish, Frog, Frog (Green Treefrog), Gerbil, Guinea Pig, Guinea Pig, Hagfish, Hamster, Human, Lizard, Mole Rat, Monkey, Mouse, Mudpuppy (Necturus Maculosus), Octopus, Pig, Rat, Salamander, Snail, Snail (Bulla Gouldiana), Squid, Trout Availability: In Stock Alternate Names: Gonadotropin-Releasing Hormone; Gonadorelin; Luliberin I; Gonadoliberin I; progonadoliberin-1; GRH, anti-LHRH Gene Symbol: GNRH1