Description The antibody has a proven strong Biotin-Streptavidin/HRP staining at a 1/8000-1/10,000 dilution in rat amygdala, cortex, and suprachiasmatic nucleus. The specificity of the antiserum was examined by soluble pre-adsorption with the peptides in question at a final concentration of 10-5 M. VIP immunolabeling was completely abolished by pre-adsorption with VIP. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: Secretin, gastric inhibitory polypeptide, somatostatin, glucagon, insulin, ACTH, gastrin 34, FMRF-amide, rat GHRF, human GHRF, peptide histidine isoleucine 27, rat pancreatic polypeptide, motilin, peptide YY, substance P, neuropeptide Y, and CGRP. The histochemical antibody for VIP is generated in a rabbit against porcine VIP conjugated to bovine thyroglobulin with carbodiimide. The antibody is provided as 100 µL of lyophilized whole serum, and 0.09% sodium azide. Photo Description: IHC image of rat cortex (above) and low magnification image of rat striatum and amygdala staining for VIP (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. Host:Rabbit Quantity / Volume: 100 µL State: Lyophilized Whole Serum Reacts With: Avian (Bird), Bat, Bubalus Bubalis (Buffalo), Bullfrog, Canine, Cat, Chick, Chicken, Dog, Dove, Fish, Fowl, Fox, Frog, Gilt (Pig), Goldfish, Guinea Pig, Hamster, Hedgehog, Hen, Human, Lizard, Mink, Mole, Mollusk, Monkey, Mouse, Mudpuppy (Necturus Maculosus), Ovine, Pig, Pigeon, Platypus, Possum, Rabbit, Raccoon, Ram, Rat, Scorpaena Porcus (Fish), Sea Lamprey, Sheep, Shrew, Snake, Sparrow, Sparus Aurata (Fish), Squirrel, Starfish, Sting Ray, Suncus Murinus, Tadpole, Turkey, Turtle, Worm (Spionidae), Zebra Finch, Zebrafish Availability: In Stock Preabsorption Control: Available at Sigma Aldrich, Catalog # V6130 Alternate Names: Vasoactive intestinal polypeptide; VIP peptides, anti-VIP Gene Symbol: VIP RRID: AB_572270 Database Links: Entrez Gene: 102254866 Bat Entrez Gene: 100217965 Bird Entrez Gene: 102407426 Buffalo Entrez Gene: 101101640 Cat Entrez Gene: 396323 Chicken Entrez Gene: 484038 Dog Entrez Gene: 102095706 Dove Entrez Gene: 102898768 Fox Entrez Gene: 373781 Frog Entrez Gene: 100735454 Guinea Pig Entrez Gene: 100773469 Hamster Entrez Gene: 7432 Human Entrez Gene: 101713745 Mole Entrez Gene: 703566 Monkey Entrez Gene: 22353 Mouse Entrez Gene: 100500718 Pig Entrez Gene: 100352512 Rabbit Entrez Gene: 100145884 Ram Entrez Gene: 117064 Rat Entrez Gene: 102066375 Sparrow Entrez Gene: 101977899 Squirrel Entrez Gene: 102457597 Turtle Entrez Gene: 795653 Zebrafish