Description The ImmunoStar serotonin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/5000 – 1/10,000 in PBS/0.3% Triton X-100 – Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 100 µg of serotonin/BSA conjugate. Photo Description: Low magnification of IHC image of neurons staining for the 5-HT goat antibody in the pontine region of the rat brain (top), and high magnification of the raphe nucleus (bottom). The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% rabbit serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:7500 for 48 hours, rabbit anti-goat secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. Host: Goat Quantity / Volume: 100 µL State: Lyophilized Whole Serum Availability: In Stock Reacts With: Cat, Cod, Cow, Crayfish, Dolphin, Fish, Frog (Xenopus Laevis), Guinea Pig, Hamster, Human, Mexican salamander (Ambystoma Mexicanum), Monkey, Moth, Mouse, Pig, Rabbit, Ram (Sheep), Rat, Salamander, Snail, Tapeworm, Worm, Zebrafish Alternate Names: anti-5-HT Goat