Description The ImmunoStar FMRF-Amide antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/1,000 – 1/2,000 in PBS/0.3% Triton X-100 – biotin/avidin-HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 100 µg/mL of FMRF-Amide. Photo Description: IHC images of FMRF-amide staining in the rat hypothalamus with nickel preparatio (above) and in the dorsal horn of the rat spinal cord with standard biotin-streptavidin/HRP and DAB chromogen (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning for the hypothalamus, and frozen section for the spinal cord. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. Host: Rabbit Quantity / Volume: 100 µL State: Lyophilized Whole Serum Reacts With: Abalone (Sea Snail), Acoela (Flatworm), Annelida (Segmented Worm), Anthomedusae, Aplysia (Sea Slug), Aplysia Californica (Sea Slug), Bat, Beetle, Brachiopods, Brown Trout (Fish), Bullfrog, Caenogastropod, Caenogastropod, Carausius Morosus (Insect), Carp (Fish), Catfish, Centipede, Chiton, Cichlid Fish, Clione Limacina Pteropod (Fruit Bat), Cockroach, Crab, Crayfish, Crocodile, Crustacean, Decapod Crustacea, Dermophis Mexicanus Mexican Caecilian (Amphibian ), Diploptera Punctata (Beetle Cockroach), Earwig / forcep bug, Fish, Fly, Frog (Xenopus Laevis), Frog (Xenopus Tectum), Gastropod (Slug), Gerbil, Hagfish, Hagfish, Helix Pomatia (Snail), Hermit Crab, Human, Idotea Emarginata (Isopod), Ilyanassa Obsoleta (Sea Snail), Jellyfish, Lamprey, Leech, Lepidodasy, Lepismachilis Y-Signata, Lizard, Lobster, Locust, Locusta Migratoria (Locust), Lymnaea Stagnalis (Snail), Macrobiotus (Water Bear), Manduca Sexta (Moth), Marine, Marine Gastrotrichs , Micro-Organisms, Mollusc, Mollusca, Mollusk, Monkey, Mopalia Muscosa (Mossy Chiton), Moth, Mouse, Mussel, Mussel (Mytilus Trossulus), Octocorallia (Soft Coral), Phascolion Strombus (Sipuncula), Phestilla Sibogae (Sea Slug), Phyllodoce Maculata (Phyllodocidae), Planarian , Pleurobranchaea Japonica (Sea Slug), Polyplacophora (Chitons), Pseudaletia Unipuncta (Moth), Pygmy Squid, Ragworm, Rat, Ray, Rhodnius Prolixus (Bug), Salamander (Urodele), Sea Cucumber, Sea Pansy, Sea Slug, Shark, Shrew, Skark, Slug, Snail, Spadella Cephaloptera (Arrow Worm), Spionid Polychaete, Three-Spined Stickleback (Fish), Tick, Trout, Worm (Digenea), Worm (Eisenia Fetida), Worm (Lumbricus Terrestris), Zebrafish Availability: In Stock Alternate Names: Anti-FMRF