The ImmunoStar VIAAT antiserum was quality control tested using standard immunohistochemical methods in rat brain and spinal cord using biotin/avidin-HRP techniques.Specificity of the antiserum was demonstrated by soluble pre-adsorption and Western blot. Tissue staining is completely eliminated by pretreatment of the diluted antibody with an excess of rat VIAAT peptide residues 511-525. Photo Description: Low magnification IHC image of the rat globus pallidus staining for VIATT (above). The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. Western blot analysis of immunoprecipitated rat brain homogenates demonstrates a dense immunoreactive band of approximately 57 kD and a minor band of approximately 36 kD. Host: Rabbit Quantity / Volume: 100 µL State: Lyophilized Whole Serum Reacts With: Rat Availability: In Stock Alternate Names: Solute carrier family 32 member 1; GABA and glycine transporter; Vesicular GABA transporter; Vesicular inhibitory amino acid transporter; solute carrier family 32 (GABA vesicular transporter), member 1; VGAT / SLC32A1; rGVAT; Vgat, anti-Vgat, anti-VIAAT Gene Symbol: Slc32a1