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Description
The ImmunoStar Mu Opioid Receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat caudate putamen and spinal cord (dorsal horn) using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/6000 – 1/10000 in PBS/0.3% Triton X-100 – Biotin/avidin-HRP Technique.
Preadsorption with MOR peptide (384-398) at 10 µg/ml completely eliminates labeling. The specificity of the antiserum was determined by immunolabeling of transfected cells and immunoisolation studies.
Photo Description: IHC image of mu opioid receptor staining in the rat striatum. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:10000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Host: Rabbit
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Cat, Chicken, Frog, Guinea Pig, Human, Monkey, Mouse, Rat, Starling, Tadpole
Availability: In Stock
Alternate Names: MOR-1; Mu-type opioid receptor; M-OR1; Mu opiate receptor; Opioid receptor B; MUOR1; M-OR-1; MORA; oprm; opioid receptor, mu 1, anti-MOR
Gene Symbol: Oprm1