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经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells, Jurkat cells, mouse pancreas, rat brain, rat pancreas, HEK-293 cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, CHO cells, HSC-T6 cells. NIH/3T3 cells, Pig brain, Rabbit brain, Mouse brain, Chicken brain |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in |
human kidney tissue, human brain tissue, human colon cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | MDCK cells, HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy ACTB Ready-To-Use IHC Kit. ACTB primary antibody included.
推荐稀释比
| APPLICATION | DILUTION |
|---|---|
| Western Blot (WB) |
WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) |
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) |
IHC : 1:20-1:2000 |
| Immunofluorescence (IF)-P |
IF-P : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC |
IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) |
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |