Stimulation is required to allow detection of the COX2 protein in some cell lines and tissues. It is better to use a positive control side by side when testing.
Rat species is recommended based on IHC result, we do not guarantee WB, IP and ICC/IF for Rat.
For western blot application, this antibody has low sensitivity issue. We suggest optimizing experimental protocols (increasing lysate amount, using lower antibody dilution or higher sensitivity ECL substrate) to improve results. We also recommend ab283574 as an alternative for testing COX2 in western blot.
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