经过测试的应用
Positive WB detected in HEK-293 cells, A549 cells, C6 cells, mouse colon tissue, Caco-2 cells, RAW 264.7 cells, SMMC-7721 cells, HeLa cells, HepG2 cells, Jurkat cells, mouse brain tissue, rat brain tissue, NIH/3T3 cells, mouse liver tissue, rat kidney tissue, rat spleen tissue, rat liver tissue
Positive IHC detected in human colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in MDCK cells
Planning an IHC experiment? We recommend our IHCeasy ACTB Ready-To-Use IHC Kit. ACTB primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
APPLICATION DILUTION
Western Blot (WB) WB : 1:4000-1:10000
Immunohistochemistry (IHC) IHC : 1:50-1:500
Immunofluorescence (IF)/ICC IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.